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Verticillium Wilt Registration

As of December 2020, Sero-X is now registered for use in cotton for the reduction in formation of the microsclerotia of verticillium dahliae assisting in the management of verticillium wilt.


For the control or suppression of green mirids, silver leaf white fly (biotype b) and heliothis in cotton and suppression of diamondback moth in brassicas as specified by the Directions for Use table.

Also for use in cotton for the reduction in formation of the microsclerotia of Verticillium dahliae assisting in the management of Verticillium wilt.

APVMA Approval No:


The active constituent in Sero-X is an extract of Clitoria ternatea (Butterfly Pea). The bioactive compounds in Butterfly Pea are a group of ultra stable cyclic peptides that have distinct biological activities and perform different roles. Over 50 bioactive peptides have been identified and they are chemically diverse depending on which part of the plant they come from e.g. leaves, stems or seed etc.

Butterfly Pea cyclotides are chemically diverse – the genome of Butterfly Pea has been mapped with over 60 Cyclotide sequences having now been observed with 39 new cyclotides having been discovered during the process.

Butterfly Pea organs express different cyclotides

When we test extracts from different organs:

  1. Those from aerial parts interact with insect-like membranes to disrupt them and inhibit microsclerotia development of verticillium dahliae
  2. Those from parts that contact soil are effective against early-stage nematodes
  3. Work continues to identify the specific cyclotides responsible for fungicidal activity


Research strategy focused on the inhibition of microsclerotia development on infected plant tissue returning to the soil

  • Replicated laboratory assays began in 2016-17 – these assays demonstrated suppression of microsclerotia at all rates, whether applied to plates as a spray or drench.
  • Replicated field trials commenced 2017-18 – applications were in different regions and were
    targeted to prevent microsclerotia developing in infected plant tissue.

These field trials were conducted by Dr Karen Kirkby and team at NSW DPIE over 2 seasons and across 2 valleys. The data presented here is a summary of Dr Kirkby’s work.

Foliar sprays were applied at intervals by a ground rig or by aircraft to foliage

  • December or when majority of plants are between first square and first flower.
  • February or when majority of plants are between mid to late flowering.
  • March or with first defoliation.

Measurements of inoculum levels (propagules per gram) were taken pre-plant and again during the growing season and post-harvest.


In seasons where PPG significantly increases Sero-X will limit the increase and in season where there would be a natural reduction, Sero-X can speed up the reduction by limiting the amount of infected tissue returning to the soil.

For trial data graphs and results please see PDF label update below.


Field and laboratory trial data confirm that Sero-X Pesticide containing 400g/L Clitora ternatea extract, provides effective suppression of microsclerotia of Verticillium dahliae in cotton and would assist in management of verticillium wilt as an alternative to crop rotation.

For more technical information and trial results, please view the Sero-X Verticillium Wilt Label Update technical document which can be found by clicking the green button located at the top of this page.

Alternatively you can contact us on 02 6795 3787 or email [email protected]